Clean Air Initiative: GlobalClean Air Initiative: AsiaIniciativa del Aire Limpio: América LatinaClean Air Initiative: Sub-Saharan Africa
Advanced Search
Countries
Topics
CAI Listserv
Air Quality Newsletters
Opportunities

Better Air Quality (BAQ) 2008
was held in Bangkok, Thailand
12-14 November 2008

Join the CAI-Asia Partnership

Time Course of Gene Expression of Inflammatory Mediators in Rat Lung after Diesel Exhaust Particle Exposure
K. Murali Krishna Rao, Jane Y. C. Ma, Terence Meighan, Mark W. Barger, Donna Pack, and Val Vallyathan

Abstract

Diesel exhaust particles (DEPs) at three concentrations (5, 35, and 50 mg/kg body weight) were instilled into rats intratracheally. We studied gene expression at 1, 7, and 30 days postexposure in cells obtained by bronchoalveolar lavage (BAL) and in lung tissue. Using real-time reverse transcriptase-polymerase chain reaction (RT-PCR), we measured the mRNA levels of eight genes [interleukin (IL)-1â, IL-6, IL-10, iNOS (inducible nitric oxide synthase), MCP-1 (monocyte chemoattractant protein-1), MIP-2 (macrophage inflammatory protein-2), TGF-â1 (transforming growth factor-â1), and TNF- (tumor necrosis factor-)] in BAL cells and four genes [IL-6, ICAM-1 (intercellular adhesion molecule-1), GM-CSF (granulocyte/macrophage-colony stimulating factor), and RANTES (regulated upon activation normal T cell expressed and secreted)] in lung tissue. In BAL cells on day 1, high-dose exposure induced a significant up-regulation of IL-1â, iNOS, MCP-1, and MIP-2 but no change in IL-6, IL-10, TGF-â1, and TNF- mRNA levels. There was no change in the mRNA levels of IL-6, RANTES, ICAM-1, and GM-CSF in lung tissue. Nitric oxide production and levels of MCP-1 and MIP-2 were increased in the 24-hr culture media of alveolar macrophages (AMs) obtained on day 1. IL-6, MCP-1, and MIP-2 levels were also elevated in the BAL fluid. BAL fluid also showed increases in albumin and lactate dehydrogenase. The cellular content in BAL fluid increased at all doses and at all time periods, mainly due to an increase in polymorphonuclear leukocytes. In vitro studies in AMs and cultured lung fibroblasts showed that lung fibroblasts are a significant source of IL-6 and MCP-1 in the lung.

Read paper at Environment Health Perspective

chemokines, diesel, lung, molecular biology, monocyte/macrophage
Quick Links

Who we are:
- CAI-Asia Center
- CAI-Asia Partnership
- Country Networks

Key documents:
- Annual Report 2008
- Fuel Roadmap
- Country Synthesis Reports
- Compendium | CitiesACT.org
- Benchmarking Report
- Quarterly Report (Center)
- Newsletters
................................more >>

Related Topics
Measuring impacts > Health impacts
Conventional fuels > Diesel
Authors
Environmental Health Perspectives

Secretariat: CAI-Asia Center, 3510 Robinsons Equitable Tower, ADB Ave., Ortigas Center, Pasig City, Philippines 1605
Tel: +632 3952843 to 45 / Fax: +632 3952846